Post-Conference Short Courses

 Friday, September 28th
 

Introduction and applications of the SPM technique
 

Speakers:
Ing. Carlos Segovia (coordinator). Specialist in SPM Micra/Veeco (topics A to E)
Dr. Sergei Manganov. Researcher of Veeco (topics F, G and I)
Dr. Luis Felipe Jimenez UNAM science school (topic H)
 
Topics:

1. Introduction: SPM and the nanotechnoloy

2. Brief history of the technique

3. Comparison between SPM and other microscopies

4. An SPM equipment part by part

5. SPM, something more than images: Differents scanning modes

6. Applications in the material sciences

7. Applications in polymers

8. Applications in biological sciences

9. New developments in SPM

10. Practices

11. Sample conditions requirements

12. Selection of cantilevers and tips

13. Image production

Electron microscopy of pathogenic protozoan
 
Speakers:
Dr. Wanderley de Souza (coordinator)
Dr. Thais Souto-Padrón
Dr. Márcia Attias
 
Topics:

1. Scanning electron microscopy: Principles and applications

2. Conventional transmission electron microscopy: Principles and applications

3. Special techniques in transmission electron microscopy

4. Electron microscopy of tripanosomatides

5. Electron microscopy of apicomplex

6. Electron microscopy of anaerobic protozoan  
    

Preparation of Odontological Samples for MEB
 
Speakers:
Dra. Graciela Durso
Odont. Adrian Abal
Prof. Patricia Sarmiento
     
Objective
General description of techniques for the processing of biological samples.
General protocol: demineralization, fixation, dehydration, drying, assembly and metallizing. Types of fixation agents, criteria for selection.
Types of dehydrating agents: Why I must eliminate the water of the samples?
Drying; types of drying: evaporation or drying by Critical Point; description of the Critical Point method.
Adhesives and Assembly: characteristics, types and selection according to the sample type
   
 Agenda
   1. Description of Tissues of the Tooth and Paradentary tissues. Hard and soft tissues.            Organic and inorganic components. Characteristics to consider for the preparation             of Odontological Samples
   2. Fracture Techniques
   3. Inclusion and abrasion Techniques
   4. Demineralization Techniques
   5. Replica Techniques
   6. Metallizing: can we observe samples without metallizing? ,
            What are the metallizing, devices, possible, electrostatic charges
   7. Areas of application
 
 
  
Basic principles and applications Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM)
 
Speaker:
Dr Mario Miki Yoshida     
 
Objective
Description of the main components of the Scanning Electron Microscope and Transmission Electron Microscope with the purpose to understand the principles and different modes of  operation and to illustrate the use of these equipment and some of its applications in materials science.
 
Agenda
A. Main components of the electron microscopes
         1. Introduction
         2. Vacuum System
         3. Electron gun
         4. Electromagnetic lenses
         5. Detectors
B. Scanning Electron Microscopy (SEM)
         1. Introduction
         2. SEM principles of operation
         3. Interaction of the electron beam with the sample
         4. Secondary and backscattered Electrons
         5. Volume of interaction
         6. Resolution and depth of field
         7. Contrast and quality of signal
         8. Applications
 C. Transmission Electron Microscopy (TEM)
         1. Introduction
         2. Illumination system, objective lenses and image system
         3. Images and diffraction patterns
         4. Modes of operation:
               1. Conventional transmission
               2. Scanning transmission (STEM)
         5. Mass-thickness contrast, Z contrast, phase contrast
         6. Other techniques
         7. Applications